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Catalog Number | orb1927843 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB18046 |
Tested applications | IF, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 82536 Da |
Target | This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-128 amino acids from the N-terminal region of human CHAT. |
UniProt ID | P28329 |
NCBI | NP_066265.3, NP_066266.3, NP_065574.3, NP_001136406.1, NP_001136405.1, NP_066264.3, NP_001136401.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Choline O-acetyltransferase, CHOACTase, ChAT, Chol Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of CHAT Antibody (N-term) in NCI-H460 cell line lysates (35 ug/lane).CHAT (arrow) was detected using the purified Pab.
Western blot analysis of CHAT Antibody (N-term) in mouse liver tissue lysates (35 ug/lane). CHAT (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of CHAT Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).