CFLAR Antibody

• Catalog Number: orb1239384
• Category: Antibodies
• Description: CFLAR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: FLIP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human FLIP.The immunogen is located within amino acids 180 - 230 of FLIP.
• Concentration: 1 mg/ml
• Dilution range: FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa and 25 kDa
• Target: CFLAR
• UniProt ID: O15519
• NCBI: AAC51622
• Storage: FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: FLIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Cas
• Note: For research use only
• Application notes: FLIP can be used for detection of FLIP by Western blot at 1:1000 dilution. 35 kDa and 25 kDa bands can be detected. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FLIP in total cell lysates from HeLa (H), Jurkat (J), THP-1 (T), A431 (A), K562 (K) and NIH3T3 (N) cells with FLIP antibody at 1:1000 dilution.

Immunocytochemistry staining of HeLa cells using FLIP antibody at 2 µg/mL.

Immunofluorescence of FLIP in HeLa cells with FLIP antibody at 10 µg/mL.

Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 20 µg/mL. Red: FLIP Antibody (orb1239384) Blue: DAPI staining.

Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 2 µg/mL.