You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1238926 |
---|---|
Category | Tools |
Description | CFLAR Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, WB |
Isotype | IgG |
Immunogen | FLIP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human FLIPaFLIPl form.The immunogen is located within the last 50 amino acids of FLIP. |
Concentration | 1 mg/ml |
Dilution range | Flip antibody can be used for detection of FLIP by Western blot at 1 μg/mL. A 55 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 55 kDa |
Target | CFLAR |
UniProt ID | O15519 |
NCBI | AAC51622 |
Storage | FLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | FLIP Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | FLIP Antibody: CASH, FLIP, MRIT, CLARP, FLAME, Cas Read more... |
Note | For research use only |
Application notes | Flip antibody can be used for detection of FLIP by Western blot at 1 μg/mL. A 55 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of FLIP in HeLa (H), Jurkat (J), and K562 (K) whole cell lysate with FLIP antibody at 1:1000 dilution.
Immunocytochemistry of FLIP in Jurkat cells with FLIP antibody at 10 µg/mL.
Immunofluorescence of FLIP in Jurkat cells with FLIP antibody at 10 µg/mL.
Immunofluorescence of FLIP in K562 cells with FLIP antibody at 5 µg/mL. Red: FLIP Antibody (orb1238926) Blue: DAPI staining.
WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating