CES1 Antibody

• Catalog Number: orb1246741
• Category: Antibodies
• Description: CES1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NTQAAQKLKDKE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: CES1
• UniProt ID: P23141
• NCBI: NP_001020366.1, NP_001020365.1, NP_001257.4
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CES1, carboxylesterase 1 (monocyte/macrophage seri
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 55-60kDa band observed in Human Liver and Ileum lysates (calculated MW of 62.6kDa according to NP_001020366.1). In transfected HEK293 transiently expressing CES1 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Lung shows staining of lymphocytes in the vasculature.. Recommended concentration, 2-3ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246741 (0.03 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246741 (2.5 ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.