CENP-A Antibody

• Catalog Number: orb67389
• Category: Antibodies
• Description: Mouse Anti-Human CENP-A Monoclonal IgG1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5A7-2E11
• Tested applications: ICC, IF, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Synthetic peptide corresponding to a portion of human CENP-A
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: Unconjugated
• MW: 18kDa
• Target: CENPA
• Entrez: 1058
• UniProt ID: P49450
• NCBI: NP_001035891.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: CENP A Antibody, cenpa Antibody, Centromere auto a
• Note: For research use only
• Application notes: 1 µg/ml of SMC-202 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Western Blot analysis of Human U2OS cell lysate showing detection of CENP-A protein using Mouse Anti-CENP-A Monoclonal Antibody, Clone 5A7-2E11. Primary Antibody: Mouse Anti-CENP-A Monoclonal Antibody at 1:1000.