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Catalog Number | orb345606 |
---|---|
Category | Antibodies |
Description | Cenexin-1 (phospho-S796) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Monkey, Primate |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near S796 of human cenexin-1. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 - 1:10,000, IHC: User Optimized, WB: 1:200 - 1:2,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phospho-peptide coupled to agarose beads followed by solid phase adsorption against nonphospho-peptide. This antibody is specific for human Cenexin-1 protein phosphorylated at S796. A BLAST analysis was used to suggest cross-reactivity with Cenexin-1 from human, chimpanzee and macaque based on 100% homology with the immunizing sequence. Cross-reactivity with Cenexin-1from other sources has not been determined. |
Conjugation | Unconjugated |
UniProt ID | Q5BJF6 |
NCBI | NP_702915 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-Cenexin-1 pS796 Antibody, ODF84 antibo Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to phosphorylated Cenexin-1 by western blotting in the appropriate cell lysate or extract. This phospho-specific polyclonal antibody reacts with human Cenexin-1 pS796 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. |
Expiration Date | 12 months from date of receipt. |
ELISA of Rabbit Anti-Cenexin-1 pS796 Antibody. Antigen: BSA conjugates of Cenexin-1 pS796 (purple) and S796 (blue). Coating amount: 0.1 µg per well. Primary antibody: Cenexin-1 pS796 at 5 µg/mL. Secondary antibody: Peroxidase goat anti-rabbit secondary antibody at 1:8500. Substrate: TMB.
Western Blot of Rabbit Anti-Cenexin-1 pS796. Lane 1: MW. Lane 2: Human Semen Lysate. Lane 3: MCF7 WCL (p/n orb348664). Lane 4: Molt 4 WCL (p/n orb348696). Lane 5: HeLa WCL (p/n orb348668). Load: 10 µg per lane. Blocking: (p/n orb348644). Primary Antibody: Anti-Cenexin-1 pS796 at 1 µg/mL overnight at 2-8°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) 1:40000 diluted in (p/n orb348644) for 30 minutes at RT. Expect: 93 kDa.
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