CELA3B Antibody / Elastase 3B

• Catalog Number: orb750077
• Category: Antibodies
• Description: This mAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein; it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CELA3B/1757
• Tested applications: ELISA, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant partial protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CELA3B antibody is available for research use only.
• UniProt ID: P08861
• Storage: Store the CELA3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CELA3B antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC testing of human pancreas with CELA3B antibody (clone CELA3B/1757). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE Analysis of Purified, BSA-Free CELA3B Antibody (clone CELA3B/1757). Confirmation of Integrity and Purity of the Antibody.