CEACAM1 Antibody

• Catalog Number: orb1566959
• Category: Antibodies
• Description: CEACAM1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Mouse
• Immunogen: Residues 35-141 (ECD)
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1,000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CEACAM1
• UniProt ID: P31809
• NCBI: NP_001034274.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Mhv-1; Cea7; CEA-related cell adhesion molecule 1;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse CEACAM1 by western blot. Samples: Whole cell lysate (25 µg) from TCMK-1, CH27 (10 µg) , NIH 3T3, WEHI-231, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (orb1566959) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CEACAM1 (shaded) in CH27 cells by flow cytometry. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (orb1566959) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.