CDX2 Antibody

• Catalog Number: orb1710843
• Category: Antibodies
• Description: CDX2 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ICC, IHC, WB
• Reactivity: Human
• Immunogen: CDX2
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with pressure cooker using citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with pressure cooker using citrate buffer pH 6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CDX2
• UniProt ID: Q99626
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: caudal-type homeobox protein 2; CDX2/AS; CDX3; CDX
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CDX2 by western blot. Samples: Whole cell lysate (50 µg) from HCT 116, DLD-1, HEK293T, HT-29, and SW620 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CDX2 monoclonal antibody [BC39] (orb1710843) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CDX2 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Mouse anti-CDX2 monoclonal antibody [BC39]. Secondary: HRP-conjugated goat anti-mouse IgG.

Detection of human CDX2 by immunocytochemistry. Sample: FFPE section of DLD-1 cells. Antibody: Mouse anti-CDX2 monoclonal antibody [BC39] (orb1710843). Secondary: HRP-conjugated goat anti-mouse IgG.