CDKN2A (phospho-S326) antibody

• Catalog Number: orb235087
• Category: Antibodies
• Description: Rabbit polyclonal antibody against CDKN2A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S326 of human p16 INK4a protein. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CDKN2A
• Entrez: 1029
• UniProt ID: P42771
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-ARF antibody, anti-CCM2 antibody, anti-CDK4 i
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of p16 INK4a (Phospho-S326) expression in HeLa UV-treated (A) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 16 kD)

Immunohistochemical analysis of p16 INK4a (Phospho-S326) staining in human cervix cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.