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Catalog Number | orb1239037 |
---|---|
Category | Antibodies |
Description | CDKN2A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Anti-CDKN2A antibody (orb1239037) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CDKN2A. The immunogen is located within the last 50 amino acids of CDKN2A. |
Concentration | 1 mg/mL |
Dilution range | WB: 1 - 2 μg/mL; IHC: 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 17kDObserved: 17kD |
Target | CDKN2A |
UniProt ID | P42771 |
NCBI | NP_000068 |
Storage | CDKN2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | CDKN2A Antibody: ARF, MLM, P14, P16, P19, CMM2, IN Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
KO Validation of CDKN2A in 293 Cells. Loading: 10 µg of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, orb1239037 (2 µg/mL) and beta-actin, orb1240312 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CDKN2A, orb1239282 (2 µg/mL), CDKN2A, orb1239037 (2 µg/mL) and beta-actin, orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CDKN2A, orb1239037 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of CDKN2A in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-CDKN2A antibody (orb1239037) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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