CDC42 Antibody

• Catalog Number: orb546275
• Category: Antibodies
• Description: CDC42 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CDC42 recombinant protein (Position: K16-C188).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 21 kDa
• UniProt ID: P60953
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cell division control protein 42 homolog; G25K GTP
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-CDC42 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U20S cells using anti-CDC42 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-CDC42 antibody.Lane 1:human placenta tissue;2:U937 cell;3:HL-60 cell;4:U20S cell;5:U87 cell;6:K562 cell;7:HeLa cell;8:rat thymus tissue;9:rat lung tissue;10:mouse lung tissue;11:mouse intestines tissue;12:mouse SP2/0 cell.

IHC analysis of CDC42 using anti-CDC42 antibody. CDC42 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of CDC42 using anti-CDC42 antibody. CDC42 was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of CDC42 using anti-CDC42 antibody. CDC42 was detected in paraffin-embedded section of rat intestine tissue.

IHC analysis of CDC42 using anti-CDC42 antibody. CDC42 was detected in paraffin-embedded section of mouse intestine tissue.