Cdc25B rabbit pAb

• Catalog Number: orb771013
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cdc25B
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDC25B. AA range:289-338
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 65
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CDC25B antibody, anti-CDC25HU2 antibody, anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HUVEC cells, using CDC25B Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from Raw264.7 cells, treated with Hu 2nM 24h, using CDC25B Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using CDC25B antibody.