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CDC25B Antibody (Center)
Catalog Number: orb1929430
| Catalog Number | orb1929430 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB07936 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:2000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 64987 Da |
| Target | This CDC25B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-360 amino acids from the Central region of human CDC25B. |
| UniProt ID | P30305 |
| NCBI | NP_068659.1, NP_004349.1, NP_068658.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | M-phase inducer phosphatase 2, Dual specificity ph Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDC25B antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Anti-CDC25B (S353) Antibody at 1:2000 dilution + Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 63 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-CDC25B Antibody (Center) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: HT-29 whole cell lysate. Lane 4: mouse lung lysate. Lane 5: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
