CDC25A Antibody

• Catalog Number: orb1261160
• Category: Antibodies
• Description: CDC25A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 235-484 of human CDC25A (NP_963861.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 59kDa
• Target: CDC25A
• UniProt ID: P30304
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: CDC25A, CDC25A2-CAG isoform, Cell division cycle 2
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CDC25A antibody (orb1261160) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat liver using CDC25A antibody (orb1261160) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver damage using CDC25A antibody (orb1261160) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibody (orb1261160) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using CDC25A Polyclonal Antibody (orb1261160) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.