CD99 Antibody / MIC2

• Catalog Number: orb1822569
• Category: Antibodies
• Description: Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MIC2/7861
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2, kappa
• Immunogen: A recombinant partial protein sequence (within amino acids 1-185) from the human protein was used as the immunogen for the CD99 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD99 antibody is available for research use only.
• UniProt ID: P14209
• Storage: Aliquot the CD99 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human pancreas tissue with CD99 antibody (clone MIC2/7861). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreas tissue with CD99 antibody (clone MIC2/7861). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human MCF-7 cell lysate with CD99 antibody (clone MIC2/7861). Predicted molecular weight: 16-32 kDa depending on the level of glycosylation.

SDS-PAGE analysis of purified, BSA-free CD99 antibody (clone MIC2/7861) as confirmation of integrity and purity.