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CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Catalog Number: orb1816877

DispatchUsually dispatched within 5-10 working days
$ 270.00
Catalog Numberorb1816877
CategoryAntibodies
DescriptionCD90/Thy-1 Recombinant Rabbit Monoclonal Antibody
Species/HostRabbit
ClonalityRecombinant
Clone Number4C6
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rat
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CD90/Thy-1
Concentration1mg/ml
Dilution rangeWB=1:1000-5000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
Form/AppearanceLiquid
ConjugationUnconjugated
MW12 kDa
TargetTHY1
UniProt IDP04216
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesCD90 / Thy1; CD7; CD90 antigen; CDw90; FLJ33325; M
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NoteFor research use only
Expiration Date12 months from date of receipt.
CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

ICC staining of THY1 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816877, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

ICC staining of THY1 in MCF-7 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816877, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

ICC staining of THY1 in PC-12 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1816877, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue).

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1816877, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1816877, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1816877, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1816877, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1816877, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue using anti-THY1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (orb1816877, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Thymus tissue lysates, Primary: Anti-CD90/Thy-1 (orb1816877) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 12 kDa, Observed band size: 25 kDa.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human cerebrum, Section type: Formalin-fixed & Paraffinembedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1816877.

CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody

Tissue: Human colon cancer, Section type: Formalin-fixed & Paraffinembedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1816877.

  • CD90/Thy-1 Recombinant Rabbit Monoclonal Antibody [orb704497]

    IF,  IHC-Fr,  IHC-P,  WB

    Rat

    Human, Rat

    Rabbit

    Recombinant

    Unconjugated

    100 μl, 50 μl