CD9 Antibody

• Catalog Number: orb606545
• Category: Antibodies
• Description: CD9 is a type IV transmembrane glycoprotein with four transmembrane domains. CD9 on pre-B cells may play a role in cell-cell adhesion. In addition, CD9 may play a role in signal transduction mediated by interaction with low molecular weight GTP binding proteins. CD9 is expressed on early B cells, eosinophils, basophils and activated T cells and is a major component of the platelet cell surface. It is also expressed on most non-T acute lymphoblastic leukemia cells and on some acute myeloid and chronic lymphoid leukemias.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD9/1619
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CD9 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD9 antibody is available for research use only.
• UniProt ID: P21926
• Storage: Store the CD9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the CD9 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Flow cytometry staining of human MCF7 cells with CD9 antibody (clone CD9/1619); Gray=unstained, Red= CD9 antibody.

FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray = unstained, Orange = CF568 labeled CD63 antibody (clone CD9/1619).

IHC staining of FFPE human basal cell carcinoma with CD9 antibody (clone CD9/1619). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human cervical carcinoma with CD9 antibody (clone CD9/1619). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human lung carcinoma with CD9 antibody (clone CD9/1619). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human tonsil with CD9 antibody (clone CD9/1619). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free CD9 antibody (clone CD9/1619) as confirmation of integrity and purity.