CD8b antibody (PE)

• Catalog Number: orb623591
• Category: Antibodies
• Description: Rat monoclonal antibody against CD8b conjugated to PE
• Clonality: Monoclonal
• Clone Number: H35-17.2
• Tested applications: FC
• Reactivity: Mouse
• Isotype: Rat IgG2b kappa
• Immunogen: Mouse CD8b
• Concentration: 0.5 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD8b
• UniProt ID: P10300
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Ly-3 antibody, anti-Ly-C antibody, anti-Lyt-3
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-4 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of murine CD8b positive splenocytes (red-filled) from murine CD8b negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8b (H35-17.2) PE antibody (concentration in sample 0.56 μg/ml).

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8b (H35-17.2) PE antibody (concentration in sample 0.56 μg/ml).