CD8A Antibody

• Catalog Number: orb699619
• Category: Antibodies
• Description: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that serves as a coreceptor for TCR recognition of MHC class I associated peptides and supports CTL activation by binding to the MHC, while making no direct contact with the peptide.CD8 is expressed on cytotoxic suppressor T cells. It is expressed as a heterodimer of CD8 occasionally positive cells were also identified within germinal centers and within the investing squamous epithelium. In other tissues, only lymphoid cells and cells of histiocyte lineage showed positive staining for CD8.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: CD8/4391R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 135-235 from the human protein was used as the immunogen for the recombinant CD8A antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant CD8A antibody is available for research use only.
• UniProt ID: P01732
• Storage: Store the recombinant CD8A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant CD8A antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with recombinant CD8A antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human lymph node with recombinant CD8A antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant CD8A antibody. These results demonstrate the foremost specificity of the CD8/4391R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.