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Catalog Number | orb1151692 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD8A |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rCD8/6590 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A portion of amino acids 135-235 of CD8 alpha protein was used as the immunogen for the recombinant CD8A antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This recombinant CD8A antibody is available for research use only. |
UniProt ID | P01732 |
Storage | Aliquot the recombinant CD8A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the recombinant CD8A antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human lymph node with recombinant CD8A antibody (clone rCD8/6590). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD8A antibody (clone rCD8/6590). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD8A antibody (clone rCD8/6590) at 2 ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CD8A antibody (rCD8/6590) as confirmation of integrity and purity.
IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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