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CD8A Antibody (N-term)
Catalog Number: orb32257
| Catalog Number | orb32257 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to CD8 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB13990 |
| Tested applications | IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | Synthetic Peptide |
| Dilution range | WB: 1:1000, IHC-P: 1:10-50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 25729 |
| Target | CD8A |
| UniProt ID | P01732 |
| NCBI | NP_001759.3, NP_741969.1, NP_001139345.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | anti CD 8 antibody, anti CD 8A antibody, anti CD 8 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-CD8A Antibody (N-term) in Hela cell line lysates (35 ug/lane). CD8A (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human tonsil tissue reacted with CD8A Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-CD8A Antibody (N-term) at 1:1000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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