CD86 Antibody

• Catalog Number: orb699623
• Category: Antibodies
• Description: Recognizes a protein of 70kDa, which is identified as CD86. CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkins disease.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C86/3716
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 66-195 from the human protein was used as the immunogen for the CD86 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD86 antibody is available for research use only.
• UniProt ID: P4208
• Storage: Store the CD86 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD86 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lung carcinoma with CD86 antibody (clone C86/3716). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Ramos cell lysate with CD86 antibody (clone C86/3716). Expected molecular weight: 38-70 kDa depending on glycosylation level.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD86 antibody (clone C86/3716). These results demonstrate the foremost specificity of the C86/3716 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CD86 antibody (clone C86/3716) as confirmation of integrity and purity.