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CD80 Recombinant Protein
Catalog Number: orb1228181
| Catalog Number | orb1228181 |
|---|---|
| Category | Proteins |
| Description | CD80 Recombinant Protein |
| Tested applications | WB |
| Tag | His Tag |
| Form/Appearance | Lyophilized |
| Purity | > 90% as determined by SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
| MW | 25.8 kDa |
| Target | CD80 |
| UniProt ID | P33681 |
| Protein Sequence | Val 35 - Asn 242 |
| Source | HEK293 cells |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, His Tag at 20ug/mL (100 ul/well),can bind Human CD28, Fc Tag with a linear of 0.2-20 ug/mL. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.01 - 0.1 ug/mL in the presence of PHA. |
| NCBI | P33681 |
| Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
| Buffer/Preservatives | PBS, pH 7.4 |
| Alternative names | CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7 Read more... |
| Note | For research use only |
| Application notes | This recombinant protein can be used for WB. For research use only. |
| Expiration Date | 6 months from date of receipt. |
Human B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- Item 1 of 2
Human B7-1 Protein, mFc-His Tag [orb757420]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
E.coli
100 μg, 50 μg, 10 μg - Item 1 of 2
Human B7-1 Protein, hFc Tag [orb689457]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 50.0 kDa after removal of the signal peptide.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 2
Mouse B7-1 Protein, hFc Tag [orb1290897]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide. The apparent molecular mass of mB7-1-hFc is approximately 70-100 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg - Item 1 of 3
B7-1 / CD80 Recombinant Protein [orb1231165]
ELISA, WB
> 95% as determined by SDS-PAGE.
50.9 kDa
HEK293 cells
0.1 mg - Item 1 of 1
