CD80 Recombinant Protein

• Catalog Number: orb1228181
• Category: Proteins
• Description: CD80 Recombinant Protein
• Tested applications: WB
• Tag: His Tag
• Dilution range: This recombinant protein can be used for WB. For research use only.
• Form/Appearance: Lyophilized
• Purity: > 90% as determined by SDS-PAGE.Less than 1.0 EU per ug by the LAL method.
• MW: 25.8 kDa
• Target: CD80
• UniProt ID: P33681
• Protein Sequence: Val 35 - Asn 242
• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, His Tag at 20ug/mL (100 ul/well),can bind Human CD28, Fc Tag with a linear of 0.2-20 ug/mL. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.01 - 0.1 ug/mL in the presence of PHA.
• NCBI: P33681
• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Alternative names: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
• Note: For research use only
• Application notes: This recombinant protein can be used for WB. For research use only.
• Expiration Date: 6 months from date of receipt.

Human B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.