CD80 Recombinant Protein

• Catalog Number: orb1228180
• Category: Proteins
• Description: CD80 Recombinant Protein
• Tested applications: WB
• Tag: C-Fc Tag
• Dilution range: This recombinant protein can be used for WB. For research use only.
• Form/Appearance: Lyophilized
• Purity: > 95% as determined by SEC-HPLC and SDS-PAGE.
• MW: 50 kDa
• Target: CD80
• UniProt ID: P33681
• Protein Sequence: Val 35 - Asn 242
• Source: HEK293 cells
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 ug/mL (100 ul/well) can bind Biotinylated Human CTLA-4 with a linear range of 50 - 500 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human B7-1, Fc Tag (HPLC-verified) at 2 ug/mL (100 ul/well) can bind Human CTLA-4, Strep Tag with a linear range of 1-20 ng/mL.
• NCBI: NP_005182.1
• Storage: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 50 mM tris, 100 mM glycine, pH 7.5
• Alternative names: CD80, B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7
• Note: For research use only
• Application notes: This recombinant protein can be used for WB. For research use only.
• Expiration Date: 6 months from date of receipt.

Human B7-1, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.