CD80 Detection Set (Risk Free)

• Catalog Number: orb1227442
• Category: Assays and Kits
• Description: CD80 Detection Set (Risk Free)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, IgG2b
• Immunogen: CD80 antibodies were raised against the extracellular domain of human CD80.
• Concentration: Antibody 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PBS containing 0.02% sodium azide.
• Note: For research use only
• Expiration Date: Please enquire.

Titration curve analysis of CD80 mAbs to detect recombinant CD80 in ELISA with orb1239279, orb1239274, orb1239278, orb1239276, orb1239275, and orb1239277 abs at decreasing concentrations.

Western blot analysis of CD80 in overexpressing HEK293 cells using orb1239275 and orb1239277 antibody at 0.25 and 0.5 μg/ml, respectively.

Immunocytochemistry of CD80 in overexpressing HEK293 cells using (A) polyclonal CD80 antibody, (B) orb1239279, (C) orb1239274, (D) orb1239278, (E) orb1239276, (F) orb1239275, (G) orb1239277 and (H) control mouse IgG antibody at 1 μg/ml.

Immunofluorescence of CD80 in overexpressing HEK293 cells using (A) polyclonal CD80 antibody, (B) orb1239279, (C) orb1239274, (D) orb1239278, (E) orb1239276, (F) orb1239275, (G) orb1239277 and (H) control mouse IgG antibody at 2 μg/ml.

Immunofluorescence of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody, (B) orb1239279, (C) orb1239274, (D) orb1239278, (E) orb1239276, (F) orb1239275, (G) orb1239277 and (H) control mouse IgG antibody at 20 μg/ml.

Immunofluorescence of CD in human tonsil tissue using (A) polyclonal CD80 antibody, (B) orb1239279, (C) orb1239274, (D) orb1239278, (E) orb1239276, (F) orb1239275, (G) orb1239277 and (H) control mouse IgG antibody at 2 μg/ml.

Immunohistochemistry of CD80 in human stomach carcinoma tissue using (A) polyclonal CD80 antibody at 1 μg/ml, (B) orb1239279, (C) orb1239274, (D) orb1239278, (E) orb1239276, (F) orb1239275, (G) orb1239277 and (H) control mouse IgG antibody at 5 μg/ml.

Immunohistochemistry of CD80 in tonsil tissue using (A) polyclonal CD80 antibody at 1 μg/ml, (B) orb1239279, (C) orb1239274, (D) orb1239278, (E) orb1239276, (F) orb1239275, (G) orb1239277 and (H) control mouse IgG antibody at 5 μg/ml.

Flow cytometry analysis of CD80 overexpressing HEK293 cells using (A) orb1239279, (B) orb1239274, (C) orb1239276, (D) orb1239275, (E) orb1239277, and (F) control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.