CD80 Antibody

• Catalog Number: orb1671561
• Category: Antibodies
• Description: CD80 Antibody
• Clonality: Recombinant
• Clone Number: 16C10
• Tested applications: Blocking, ELISA
• Reactivity: Human
• Isotype: IgG lambda
• Immunogen: This antibody was generated by immunization of cynomolgus monkeys with human B7.1 antigen.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CD80
• UniProt ID: P33681
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: B7.1, CD28L, CD28LG, B7-1, B7, BB1, B7/BB1,T-lymph
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody 16C10. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric r version of 16C10 (orb1671561) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671561- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the anti-CD80 antibody 16C10. Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of 16C10 (orb1671561 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.