CD8 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563017
• Category: Antibodies
• Description: CD8 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 9A6
• Tested applications: IF, IHC-Fr, IHC-P
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD8
• Antibody Type: Recombinant Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:50-300, IHC-F=1:50-300, IF=1:50-300
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: CD8A
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD8 antigen, alpha polypeptide(p32); CD8a; CD8a an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD8 Monoclonal Antibody, Unconjugated (orb2563017) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD8 Monoclonal Antibody, Unconjugated (orb2563017) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Small Intestine, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD8 Monoclonal Antibody, Unconjugated (orb2563017) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD8 Monoclonal Antibody, Unconjugated (orb2563017) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Thymus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD8 Monoclonal Antibody, Unconjugated (orb2563017) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD8 Monoclonal Antibody, Unconjugated (orb2563017) at 1:300 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.