CD8 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1610270
• Category: Antibodies
• Description: CD8 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: D5A7
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD8
• Antibody Type: Recombinant Antibody
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: CD8A
• UniProt ID: P01732
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Alternative names: CD8 antigen, alpha polypeptide(p32); CD8a; CD8a an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD8) Monoclonal Antibody, Unconjugated (orb1610270) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CD8) Monoclonal Antibody, Unconjugated (orb1610270) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.