CD8 Rabbit Polyclonal Antibody

• Catalog Number: orb526500
• Category: Antibodies
• Description: CD8 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD8 (51-150/247aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 27 kDa
• Target: CD8A
• UniProt ID: P01731
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CD8 antigen, alpha polypeptide(p32); CD8a; CD8a an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Blood (Mouse) Lysate at 40 ug, Primary: Anti-CD8 (orb526500) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 32kD.

Sample: Raw264.7 (Mouse) Cell Lysate at 30 ug, Primary: Anti-CD8 (orb526500) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-CD8 (orb526500) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.