CD8 antibody

• Catalog Number: orb323288
• Category: Antibodies
• Description: CD8 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD8. Please contact us for the exact immunogen sequence. The peptide is available as orb374945.
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Dilution range: IHC-P: 1:100-800, WB: 1:100-500, IF/ICC: 1:100-800
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated
• MW: 26 kDa
• Target: CD8. Recognizes endogenous levels of CD8 protein
• Entrez: 925
• UniProt ID: P01732, P07725, P01731
• NCBI: 001139345, 001145873
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mahshad Entezam 1, Nader Bagheri 2, Amin Soltani 3, Sayedeh Azimeh Hosseini 4, Pegah Khosravian 5, Gordon A Ferns 6, Mahdi GhatrehSamani 7 Enhanced antitumor immunity in breast cancer: Synergistic effects of ADAM10/ADAM17 inhibition, metabolic modulation, and camptothecin-loaded selenium nanoparticles Int J Pharm, (2024)

PubMed: 39675534

Mohammad Jahan-Mahin 1, Roya Askari 1, Amir Hossein Haghighi 1, Omid Khaiyat The effect of three types of water-based training protocols on thymus atrophy and specific indicators of cellular immune senescence in aged male rats Biogerontology, (2025)

PubMed: 39832052

Applications

IHC

Reactivity

Rat

Isocitrate dehydrogenase mutations suppress STAT1 and CD8+ T cell accumulation in gliomas J Clin Invest, 127, 1425-1437 (2017)

PubMed: 28319047

Applications

IF

Reactivity

Mouse

IHC-P image of mouse spleen tissue using CD8 antibody (2.5 ug/ml)

WB analysis of rat thymus (lane 1), mouse spleen (lane 2), rat brain (lane 3), human thyroid tumour (lane 4) using CD8 antibody (5 ug/ml)

IHC-P image of mouse spleen tissue using CD8 antibody (2.5 ug/ml)

IHC-P staining of rat thymus tissue using anti-CD8 (2.5 ug/ml)

IF image of rat thymus tissue using anti-CD8 (2.5 ug/ml)

Immunohistochemical staining of mouse lymph node tissue using CD8 antibody (2.5 ug/ml)

Western blot analysis of rat thymus (lane 1), mouse spleen (lane 2), rat brain (lane 3), human thyroid tumour (lane 4) using CD8 antibody (5 ug/ml)

IHC-P image of rat thymus tissue using anti-CD8 (2.5 ug/ml)

IHC-P image of mouse skin tissue using CD8 antibody (2.5 ug/ml)

Immunofluorescence analysis of rat thymus tissue using CD8 antibody (2.5 ug/ml)

Immunohistochemical staining of mouse skin tissue using anti-CD8 (2.5 ug/ml)

Immunohistochemical staining of mouse skin tissue using CD8 antibody (2.5 ug/ml)