CD74 / CLIP Antibody

• Catalog Number: orb750284
• Category: Antibodies
• Description: CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM523
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: SU-DHL-4 lymphoma cells were used as the immunogen for this anti-CD74 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This anti-CD74 antibody is available for research use only.
• UniProt ID: P04233
• Storage: Store the anti-CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human Raji cells with anti-CD74 antibody (clone SPM523); Red=isotype control, Blue= anti-CD74 antibody.

IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with anti-CD74 antibody (clone SPM523).

Immunofluorescent staining of human Raji cells with anti-CD74 antibody (clone SPM523, green) and Reddot nuclear stain (red).