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Catalog Number | orb749343 |
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Category | Antibodies |
Description | CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. CD74 antibody stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. antibody to CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | LN-2 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Mouse IgG1, kappa |
Immunogen | SU-DHL-4 lymphoma cells were used as the immunogen for this CD74 antibody. |
Dilution range | Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence (methanol fixed): 1-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD74 antibody is available for research use only. |
Entrez | 972 |
Storage | Store the CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent staining of human Raji cells with CD74 antibody (clone LN-2, green) and Reddot nuclear stain (red).
IHC staining of FFPE human spleen tissue with CD74 antibody (clone LN-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with CD74 antibody (clone LN-2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate with CD74 antibody (clone LN-2). Expected molecular weight: 33-43 kDa.
Flow cytometry testing of human Raji cells with CD74 antibody (clone LN-2); Red=isotype control, Blue=CD74 antibody.
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