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CD74 Antibody: RPE
Catalog Number: orb396350
| Catalog Number | orb396350 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody against CD74 conjugated to RPE . |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | 1B8 |
| Tested applications | ICC, IF, IHC, WB |
| Reactivity | Human |
| Isotype | IgG2a |
| Immunogen | Human CD74 invariant chain synthetic peptide. |
| Concentration | 1 mg/ml |
| Dilution range | WB (1:1000); ICC/IF (1:100) |
| Conjugation | RPE |
| MW | 33 kDa |
| Target | CD74 |
| Entrez | 972 |
| UniProt ID | P04233 |
| NCBI | NP_001020329.1 |
| Storage | Conjugated antibodies should be stored according to the product label |
| Buffer/Preservatives | 95.64mM Phosphate, 2.48mM MES and 2mM EDTA |
| Alternative names | DHLAG antibody, HLA DR gamma antibody, HLADG antib Read more... |
| Note | For research use only |
| Application notes | A 1:1000 dilution of SMC-267 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. |
| Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CD74 Antibody. (D) Composite.
Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.
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