CD73 Antibody

• Catalog Number: orb607134
• Category: Antibodies
• Description: The protein encoded by the NT5E gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CDLA73-1
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
• Dilution range: ELISA: order BSA-free format for coating,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD73 antibody is available for research use only.
• UniProt ID: P21589
• Storage: Store the CD73 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD73 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone CDLA73-1); Red=isotype control, Blue= CD73 antibody.

Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone CDLA73-1) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with CD73 antibody (clone CDLA73-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human 1) A431 and 2) PC-3 cell lysate with CD73 antibody (clone CDLA73-1). Predicted molecular weight: 60-70 kDa.