CD7 antibody

• Catalog Number: orb2276520
• Category: Antibodies
• Description: CD7 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-007R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P09564
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: GP40; Leu9; p41; T-cell leukemia antigen; T-cell s
• Note: For research use only
• Application notes: Positive Control: Tonsil: A moderate to strong, predominantly membranous staining of T-cells in the interfollicular zones and the germinal centres should be seen. Colon: A moderate to strong staining of the intra-epithelial T-cells in the colon mucosa should be seen. Negative Control: Tonsil/Colon: All epithelial structures should stain negative. Cellular Localization: Membranous Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Colon Strong CD7 staining of a large fraction of tumor infiltrating lymphocytes in a colorectal adenocarcinoma. Tumor glands are completely CD7 negative.

Strong membranous CD7 expression of an adenocarcinoma of the lung.

Tonsil Strong CD7 positivity of T lymphocytes mainly in the interfollicular area.