CD63 Antibody / LAMP-3

• Catalog Number: orb749918
• Category: Antibodies
• Description: This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63/LAMP-3. Its epitope is different from that of mAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63/LAMP-3 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LAMP3/968
• Tested applications: FACS, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant human full-length protein was used as the immunogen for the CD63 / LAMP-3 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This LAMP-3 antibody is available for research use only.
• UniProt ID: P08962
• Storage: Store the LAMP-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the LAMP-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human MCF7 cells with LAMP-3 antibody (clone LAMP3/968); Gray=unstained, Orange=CF555-labeled LAMP-3 antibody.

FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray =unstained, Orange =CF555-labeled LAMP-3 antibody.

Flow cytometry testing of PFA-fixed human U-87 MG cells with LAMP-3 antibody (clone LAMP3/968); Red=isotype control, Blue=LAMP-3 antibody.

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD63 / LAMP-3 antibody (LAMP3/968).

IHC: Formalin-fixed, paraffin-embedded human melanoma stained with CD63 / LAMP-3 antibody (LAMP3/968).

SDS-PAGE Analysis of Purified, BSA-Free LAMP-3 Antibody (clone LAMP3/968). Confirmation of Integrity and Purity of the Antibody.