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Catalog Number | orb606876 |
---|---|
Category | Antibodies |
Description | The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rMX-49.129.5 |
Tested applications | FACS, IHC-P |
Reactivity | Human, Mouse |
Isotype | Mouse IgG1, kappa |
Immunogen | Full length human CD63 was used as the immunogen for this recombinant CD63 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant CD63 antibody is available for research use only. |
Entrez | 967 |
Storage | Store the recombinant CD63 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD63 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry testing of PFA-fixed human U-87 MG cells with recombinant CD63 antibody (clone rMX-49.129.5); Red=isotype control, Blue= CD63 antibody.
SDS-PAGE analysis of purified, BSA-free recombinant CD63 antibody (clone rMX-49.129.5) as confirmation of integrity and purity.
IHC staining of FFPE human melanoma with recombinant CD63 antibody (clone rMX-49.129.5). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Flow cytometry testing of PFA-fixed human U-87 MG cells with recombinant CD63 antibody (clone rMX-49.129.5); Red=isotype control, Blue= CD63 antibody.
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
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