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Catalog Number | orb1495147 |
---|---|
Category | Proteins |
Description | CD62E Recombinant Protein |
Species/Host | E. coli |
Tag | His-tag |
MW | ~62kDa |
UniProt ID | P16581 |
Solubility (25°C) | PBS, 4M Urea, PH7.4 |
Protein Sequence | MWSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFACPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIP |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Buffer/Preservatives | PBS, 4M Urea, pH 7.4 |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 59.5 kDa after removal of the signal peptide. The apparent molecular mass of CD62E-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
WB | |
> 95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. | |
84.8 kDa | |
HEK293 cells |
WB | |
> 95% as determined by SDS-PAGE. | |
60 kDa | |
HEK293 cells |
Greater than 90% as determined by SDS-PAGE. | |
44.4 kDa | |
E.coli |
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