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Catalog Number | orb1231184 |
---|---|
Category | Proteins |
Description | CD52 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | Fc Tag |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by SDS-PAGE. |
MW | 27.9 kDa |
Target | CD52 |
UniProt ID | P31358 |
Protein Sequence | Gly 25 - Ser 36 |
Source | HEK293 cells |
NCBI | NP_001794.2 |
Storage | Lyophilized Protein should be stored at -20˚°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚°C or -70˚°C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Alternative names | CDW52,HE5,CD52,CAMPATH-1 antigen Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human CD52, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD52, Fc Tag at 10 ug/ml (100 uL/well) can bind Alemtuzumab with a linear range of 0.313-10 ug/ml.
ELISA, FC, IHC-Fr, IHC-P, WB | |
Human, Primate | |
Rat | |
Monoclonal | |
Unconjugated |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 28.3 kDa after removal of the signal peptide. The apparent molecular mass of CD52-mFc is approximately 33-53 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 27.3 kDa after removal of the signal peptide.The apparent molecular mass of CD52-hFc is approximately 35-40 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result. |