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Catalog Number | orb545706 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD49d conjugated to FITC |
Clonality | Monoclonal |
Clone Number | 9F10 |
Tested applications | FC |
Reactivity | Bovine, Canine, Equine, Feline, Human, Primate, Sheep |
Isotype | Mouse IgG1 kappa |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD49d |
Entrez | 3676 |
UniProt ID | P13612 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-ITGA4 antibody, anti-VLA-4 alpha antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD49d positive lymphocytes (red-filled) from human blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49d (9F10) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49d (9F10) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
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