CD49a antibody (PE)

• Catalog Number: orb1217409
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD49a conjugated to PE
• Clonality: Monoclonal
• Clone Number: TS2/7
• Tested applications: FC
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: human CTL line
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD49a
• Entrez: 3672
• UniProt ID: P56199
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-alpha 1 integrin antibody, anti-VLA-1 alpha a
• Note: For research use only
• Application notes: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Expiration Date: 12 months from date of receipt.

Separation of human CD49a positive lymphocytes (red-filled) from CD49a negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of HeLa cells stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 3 μg/ml, same as CD49a PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HeLa cell suspension.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49a (TS2/7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).