CD48 (Pan Leukocyte Marker) antibody

• Catalog Number: orb1151544
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD48 (Pan Leukocyte Marker)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD48/4784
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 59-178 was used as the immunogen for the CD48 antibody.
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD48 antibody is available for research use only.
• UniProt ID: P09326
• Storage: Aliquot the CD48 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD48 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node with CD48 antibody (clone CD48/4784). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with CD48 antibody (clone CD48/4784). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Raji cell lysate using CD48 antibody (clone CD48/4784). Expected molecular weight: 28-50 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free CD48 antibody (clone CD48/4784) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CD48 antibody (clone CD48/4784). These results demonstrate the foremost specificity of the CD48/4784 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.