CD47 antibody

• Catalog Number: orb44051
• Category: Antibodies
• Description: Mouse Monoclonal to CD47.
• Clonality: Monoclonal
• Clone Number: MEM-122
• Tested applications: FC, IHC-Fr, WB
• Reactivity: Human, Porcine, Primate
• Isotype: Mouse IgM
• Immunogen: COS-7 (African green monkey) cells
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: CD47
• Entrez: 961
• UniProt ID: Q08722
• RRID: AB_10996765
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Alternative names: Anti-CD47 antibody, anti-Integrin Associated Prote
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2 μg/ml. Western blotting: Non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Flow cytometry (surface staining) of lysed and washed porcine peripheral blood with purified anti-CD47 (MEM-122) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; cat. no. orb43568); Panel B - porcine PBMC sample stained with anti-CD47 (MEM-122). Cells in the granulocyte gate were used for analysis.

Separation of human lymphocytes (red-filled) from human CD47 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD47 (MEM-122) purified antibody (concentration in sample 4 µg/ml, GAM APC).