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Catalog Number | orb181434 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to CD47. |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Canine, Human |
Dilution range | ELISA: 1:128000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 35.2; 33.2 |
Target | CD47 |
Entrez | 961 |
Protein Sequence | SNQKTIQPPRK |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CD47 antibody, CD47 molecule antibody, IAP antibod Read more... |
Note | For research use only |
Application notes | This antibody is expected to recognize both reported isoforms |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Human Hippocampus (Lane 1) and HeLa (Lane 2) lysates using CD47 antibody
orb181434 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
orb181434 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane, cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
orb181434 (7 µg/mL) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
orb181434 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
orb181434 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
ICC, IF, IHC-P, WB | |
Guinea pig, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FA, FACS, Kinetics | |
Human | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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