CD47 Antibody / IAP / Integrin Associated Protein

• Catalog Number: orb749906
• Category: Antibodies
• Description: This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: IAP/964
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant human protein was used as the immunogen for the CD47 antibody.
• Dilution range: Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This CD47 antibody is available for research use only.
• UniProt ID: Q08722
• Storage: Store the CD47 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CD47 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of live human Jurkat cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red).

Immunofluorescent staining of PFA-fixed human MCF7 cells with CD47 antibody (green, clone IAP/964) and Reddot nuclear stain (red).

Flow cytometry staining of live human Jukat cells with CD47 antibody (clone IAP/964); Red=isotype control, Blue=CD47 antibody.

SDS-PAGE analysis of purified, BSA-free CD47 antibody (clone IAP/964) as confirmation of integrity and purity.