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Catalog Number | orb44052 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to CD47. |
Clonality | Monoclonal |
Clone Number | MEM-122 |
Tested applications | FC |
Reactivity | Human, Porcine, Primate |
Isotype | Mouse IgM |
Immunogen | COS-7 (African green monkey) cells |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | FITC |
Target | CD47 |
Entrez | 961 |
UniProt ID | Q08722 |
RRID | AB_10993976 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
Alternative names | Anti-CD47 antibody, anti-Integrin Associated Prote Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD47 positive lymphocytes (red-filled) from CD47 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD47 (MEM-122) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD47 (MEM-122) FITC antibody (20 μl reagent / 100 μl of peripheral whole blood).
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