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Catalog Number | orb749384 |
---|---|
Category | Antibodies |
Description | This antibody recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45) called CD45RO (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. CD45RO is found on mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. The CD45RO antibody shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, antibody to CD45RO is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | UCHL-1 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2a, kappa |
Immunogen | Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as the immunogen for the CD45RO antibody. |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This CD45RO antibody is available for research use only. |
Entrez | 5788 |
Storage | Store the CD45RO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD45RO antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of human tonsil stained with CD45RO antibody (UCHL-1).
FACS staining of human lymphocytes using CD45RO antibody (UCHL-1, red) and isotype control (green).
SDS-PAGE Analysis of Purified, BSA-Free CD45RO Antibody (clone UCHL-1). Confirmation of Integrity and Purity of the Antibody.
Immunofluorescent staining of FFPE human tonsil tissue with CD45RO antibody (clone UCHL-1).
Flow cytometry staining of PFA-fixed human Jukat cells with CD45RO antibody; Red=isotype control, Blue=CD45RO antibody.
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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