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    CD45 Antibody Cocktail (Leukocyte marker)

    Catalog Number: orb534345

    DispatchUsually dispatched within 5-10 working days
    $ 764.00
    Catalog Numberorb534345
    CategoryAntibodies
    DescriptionCD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
    Species/HostRabbit
    ClonalityRecombinant
    Clone NumberPTPRC/1975R + PTPRC/1783R
    Tested applicationsIHC-P, WB
    ReactivityHuman
    IsotypeRabbit IgG, kappa
    ImmunogenRecombinant human protein was used as immunogen for this recombinant CD45 antibody cocktail.
    Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
    PurityProtein A affinity chromatography
    ConjugationUnconjugated
    Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
    Hazard InformationThis recombinant CD45 antibody cocktail is available for research use only.
    UniProt IDP08575
    StorageThe recombinant CD45 antibody cocktail (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
    Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
    NoteFor research use only
    Application notesDue to variation in protocol and secondary antibody used, the recombinant CD45 antibody cocktail may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
    Expiration Date12 months from date of receipt.
    CD45 Antibody Cocktail (Leukocyte marker)

    IHC testing of recombinant CD45 antibody cocktail and FFPE human tonsil (clones PTPRC/1975R + PTPRC/1783R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

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