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Catalog Number | orb606401 |
---|---|
Category | Antibodies |
Description | CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies. |
Species/Host | Mouse |
Clonality | Recombinant |
Clone Number | rCD44v9/1459 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | An amino acid sequence from the variant 9 domain of CD44 was used as the immunogen for the recombinant CD44v9 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant CD44v9 antibody is available for research use only. |
UniProt ID | P16070 |
Storage | Store the recombinant CD44v9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD44v9 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human cervical carcinoma with recombinant CD44v9 antibody (clone rCD44v9/1459). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant CD44v9 antibody (clone rCD44v9/1459) as confirmation of integrity and purity.
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